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Biotechnology and Biomedical Engineering Microbiology Virtual Lab II Experiments

Aim

Theory

Pretest

Procedure

Simulation

Posttest

References

Feedback

Aim

Theory

Pretest

Procedure

Simulation

Posttest

References

Feedback

Triple Sugar Iron Agar

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Intermediate

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1. Alkaline slant (red) and acid butt (yellow) with or without gas production (breaks in the agar slant) is indicative of :

a: No carbohydrate fermentation Explanation

Explanation

b: Lactose/Sucrose fermentation Explanation

Explanation

c: Glucose fermentation Explanation

Explanation

d: Hydrogen Sulphide production Explanation

Explanation

2. The blackening of the slant and the breaks in the agar indicate:

a: carbon dioxide and hydrogen sulfide production respectively Explanation

Explanation

b: Hydrogen sulfide and carbon dioxide respectively Explanation

Explanation

c: No utilization of carbohydrates Explanation

Explanation

d: None of the above Explanation

Explanation

3. The visible black precipitate in TSI slant after incubation is:

a: Ferrous sulfate Explanation

Explanation

b: Copper sulfate Explanation

Explanation

c: Ferrous sulfide Explanation

Explanation

d: Lead sulfide Explanation

Explanation

4. Which of the following is true about TSI medium ?

a: It contains glucose, sucrose and lactose Explanation

Explanation

b: It contains the pH indicator phenol red Explanation

Explanation

c: it contains thiosulfate and ferrous sulfate Explanation

Explanation

d: All the above Explanation

Explanation

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