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Biotechnology and Biomedical Engineering Microbiology Virtual Lab II Experiments

Aim

Theory

Pretest

Procedure

Simulation

Posttest

References

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Aim

Theory

Pretest

Procedure

Simulation

Posttest

References

Feedback

Bacteriophage Plaque Assay for Phage Titer

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Beginner

Intermediate

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1. In the plaque assay, the plaques on the agar plate are areas where:

a: Infected bacteria have lysed Explanation

Explanation

b: Uninfected bacteria are growing Explanation

Explanation

c: Phages are growing Explanation

Explanation

d: The phages have died Explanation

Explanation

2. If the plaque assay serial dilution factor is too low:

a: There will be too few bacteria cells lysed to accurately count Explanation

Explanation

b: The plaques will fuse, lysing the entire culture Explanation

Explanation

c: Nothing will happen Explanation

Explanation

d: None of the bacteria will be lysed Explanation

Explanation

3. A plaque assay detects the number of __________

a: Bacterial auxotrophs Explanation

Explanation

b: Dental cavities Explanation

Explanation

c: Bacterial viruses Explanation

Explanation

d: Bacterial prototrophs Explanation

Explanation

4. Forty-eight (48) plaques were counted in a plate when 0.1 ml of bacteriophage-infected bacteria from 10¯⁶ dilution (10 ml culture) was plated. The original bacteriophage density was:

a: 4.8 X 10⁶ Explanation

Explanation

b: 48 X 10¯⁶ Explanation

Explanation

c: 4.8X 10¯ ⁷ Explanation

Explanation

d: 48 X 10⁷ Explanation

Explanation

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